Journal: Immunity
Article Title: Mitochondrial Membrane Potential Regulates Nuclear Gene Expression in Macrophages Exposed to Prostaglandin E2
doi: 10.1016/j.immuni.2018.10.011
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Differential gene expression analysis by qRT-PCR Total RNA was extracted with the RNAqueous-Micro Total RNA Isolation Kit, quantified using a Qubit and cDNA synthesized with the High-Capacity RNA-to-cDNA Kit following the manufacturer’s instructions (all Thermo Fisher Scientific). qPCR was performed on the QuantStudio 3 (Thermo Fisher Scientific) using the DreamTaq Green PCR Master Mix (2X) (BIORAD) and the following probe sets (all Applied Biosystems): Hprt (Mm03024075_m1), Retnla (Mm01193966_m1), Osm (Mm01193966_m1), Socs3 (Mm00545913_s1), Etv1 (Mm00514804_m1), Slc25a13 (Mm00489442_m1) and Got1 (Mm01195792_g1).Results were analyzed by the 2−ΔΔCt method using Hprt as a house-keeping gene.
Techniques: Recombinant, Plasmid Preparation, Software